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Sprache:
Englisch
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Beschreibung
This book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications.
Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process.
This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.
Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process.
This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.
This book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications.
Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process.
This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.
Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process.
This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.
Über den Autor
Dr. Claudio Ortolani was a senior consultant (now retired) in Clinical Pathology at the Hospital Dell'Angelo, Venice, Italy.
Zusammenfassung
Explains the technical details of a flow cytometer
Prepares the reader for solving potential problems in daily laboratory work
Covers all aspects of flow cytometric measurements
Inhaltsverzeichnis
Chapter 1. General Principles.- Chapter 2. Signals - Scattering.- Chapter 3. Signals - Fluorescence, Phosphorescence, Impedance, Extinction.- Chapter 4. Fluidics.- Chapter 5. Light Sources.- Chapter 6. Optical Benches.- Chapter 7. Detectors and Electronics.- Chapter 8. Signal Analysis.- Chapter 9. The Cytometric File.- Chapter 10. Data Transformation.- Chapter 11. Data Representation.- Chapter 12. Data Analysis.- Chapter 13. Standards, Setup, Calibration, and Control Techniques.- Chapter 14. Fluorochromes - Overview.- Chapter 15. Fluorochromes Suitable for Antibody Conjugation.- Chapter 16. Fluorochromes That Bind Nucleic Acids.- Chapter 17. Fluorochromes for the Study of the Cell Features.- Chapter 18. Fluorescent Proteins.- Chapter 19. Spillover and Compensation.- Chapter 20. Artifacts.- Chapter 21. Cell Sorting.- Chapter 22. Non-Conventional Flow Cytometry.- Chapter 23. Statistics: A Cytometric Point of View.
Details
Erscheinungsjahr: | 2022 |
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Fachbereich: | Allgemeines |
Genre: | Biologie, Mathematik, Medizin, Naturwissenschaften, Technik |
Rubrik: | Naturwissenschaften & Technik |
Thema: | Lexika |
Medium: | Taschenbuch |
Inhalt: |
xxix
544 S. 1 s/w Illustr. 544 p. 1 illus. |
ISBN-13: | 9783031108358 |
ISBN-10: | 3031108353 |
Sprache: | Englisch |
Einband: | Kartoniert / Broschiert |
Autor: | Ortolani, Claudio |
Auflage: | 1st edition 2022 |
Hersteller: |
Springer Nature Switzerland
Springer International Publishing Springer International Publishing AG |
Verantwortliche Person für die EU: | Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com |
Maße: | 235 x 155 x 29 mm |
Von/Mit: | Claudio Ortolani |
Erscheinungsdatum: | 17.11.2022 |
Gewicht: | 0,966 kg |