This book deals with an immportant subject in analytical chemistry and spectroscopy. It contains an essential contribution of the founder of this technique, the Nobel Prize winner M. Eigen.

1. Introduction.- References.- I FCS in the Analysis of Molecular Interactions.- 2 Fluorescence Correlation Spectroscopy of Flavins and Flavoproteins.- 3 Fluorescence Correlation Spectroscopy in Nucleic Acid Analysis.- 4 Strain-Dependent Fluorescence Correlation Spectroscopy: Proposing a New Measurement for Conformational Fluctuations of Biological Macromolecules.- 5 Applications of FCS to Protein-Ligand Interactions: Comparison with Fluorescence Polarization.- II FCS at the Cellular Level.- 6 FCS-Analysis of Ligand-Receptor Interactions in Living Cells.- 7 Fluorescence Correlation Microscopy (FCM): Fluorescence Correlation Spectroscopy (FCS) in Cell Biology.- 8 FCS and Spatial Correlations on Biological Surfaces.- III Applications in Biotechnology, Drug Screening, and DiagnosticsPart 2 FCS at the Cellular Level.- 9 Dual-Color Confocal Fluorescence Spectroscopy and its Application in Biotechnology.- 10 Nanoparticle Immunoassays: A new Method for Use in Molecular Diagnostics and High Throughput Pharmaceutical Screening based on Fluorescence Correlation Spectroscopy.- 11 Protein Aggregation Associated with Alzheimer and Prion Diseases.- IV Environmental Analysis and Monitoring.- 12 Application of FCS to the Study of Environmental Systems.- 13 Photophysical Aspects of FCS Measurements.- V New Developments and Trends.- 14 Fluorescence Correlation Spectroscopy: Genesis, Evolution, Maturation and Prognosis.- 15 ConfoCor 2 The Second Generation of Fluorescence Correlation Microscopes.- 16 Antibunching and Rotational Diffusion in FCS.- 17 Cross-correlation analysis in FCS.- 18 Cross-correlated Flow Analysis in Microstructures.- 19 Introduction to the Theory of Fluorescence Intensity Distribution Analysis.- 20 Photon Counting Histogram Statistics.- 21 High Order Autocorrelationin Fluorescence Correlation Spectroscopy.- 22 FCS in Single Molecule Analysis.