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Beschreibung
This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.
Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.
This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.
Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.
Inhaltsverzeichnis
Forensic DNA Analysis: An Overview of the Laboratory Process.- Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods.- Manual Silica-Based DNA Extractions.- Applied Biosystems(TM) PrepFiler(TM) Forensic DNA Extraction Kit (Manual and Semi-Automated via AutoMate Express(TM)).- Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation.- DNA Extraction of Bone through Demineralization.- Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ(TM) Methods.- DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards.- Yield Gel via Quantitative Gel Electrophoresis.- Quantitative PCR of Alu Repeats using PowerUp(TM) SYBR® Green Master Mix.- Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit.- QIAGEN's Investigator® Quantiplex Pro Kit.- DNA Amplification using Promega's PowerPlex® Fusion Systems (5C and 6C).- Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System.- Applied Biosystems' GlobalFiler(TM) PCR Amplification Kit.- QIAGEN's Investigator® 24plex QS and GO! PCR Amplification.- Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis.- Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer.- Likelihood Ratio Calculation using LRmix Studio.- Mitochondrial DNA Analysis.- An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints.- Detection of Latent DNA using a DNA Binding Dye.- Rapid DNA Profile Development with Applied Biosystems RapidHIT(TM) ID System.- Next-Generation Sequencing: ForenSeq(TM) DNA Signature Prep Kit with the Illumina MiSeq FGx.
Details
| Erscheinungsjahr: | 2024 |
|---|---|
| Fachbereich: | Gentechnologie |
| Genre: | Biologie, Importe |
| Rubrik: | Naturwissenschaften & Technik |
| Medium: | Taschenbuch |
| Reihe: | Methods in Molecular Biology |
| Inhalt: |
ix
431 S. 3 s/w Illustr. 92 farbige Illustr. 431 p. 95 illus. 92 illus. in color. |
| ISBN-13: | 9781071632970 |
| ISBN-10: | 1071632973 |
| Sprache: | Englisch |
| Einband: | Kartoniert / Broschiert |
| Redaktion: | Cupples Connon, Catherine |
| Herausgeber: | Catherine Cupples Connon |
| Hersteller: |
Humana
Springer US, New York, N.Y. Methods in Molecular Biology |
| Verantwortliche Person für die EU: | Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com |
| Maße: | 254 x 178 x 24 mm |
| Von/Mit: | Catherine Cupples Connon |
| Erscheinungsdatum: | 15.07.2024 |
| Gewicht: | 0,829 kg |