Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Neoglycoprotein and glycoprotein printing on a hydrogel functionalized microarray surface and incubation with labeled lectins.- Evaluation of glycan-binding specificity by glycoconjugate microarray with an evanescent-field fluorescence detection system.- Multiplex glycan bead array (MGBA) for high throughput and high content analyses of glycan binding proteins including natural anti-glycan antibodies.- Nanocube-based fluidic glycan array.- General strategies for glycan microarray data processing and analysis.- Calculating half maximal inhibitory concentration (IC50) values from glycomics microarray data using GraphPad Prism.- Synthetic plant glycan microarrays as tools for plant biology.- Mucin purification and printing natural mucin microarrays.- Bacterial microarrays for examining bacterial glycosignatures and recognition by host lectins.- Tissue glycome mapping: Lectin microarray-based differential glycomic analysis of formalin-fixed paraffin-embedded tissue sections.- Detection of autoantibodies using combinatorial glycolipid microarrays.- Glycan microarrays containing synthetic Streptococcus pneumoniae CPS fragments and their application to vaccine development.- Lectin-based protein microarray for the glycan analysis of colorectal cancer biomarkers: the insulin-like growth factor system.- Bacterial staining and profiling for glycan interactions on glycan microarrays for t-test calculation.- Preparation and fluorescent labelling of cell-derived micelles and profiling on glycan microarrays.