Since the early days, the field of neuropeptide biology has dramatically widened, and today the ultimate frontiers in neuropeptide research lie in the development of pharmacologically active compounds that are capable of crossing the blood-brain barrier to exert their biological role(s) in vivo and in the construction of genetic vectors to be employed in gene therapy. Neuropeptides: Methods and Protocols presents a readily reproducible collection of established and emerging techniques for neuropeptide research as contributed by expert researchers in the field. The detailed methods presented cover subjects such as immunocytochemical localization, biochemical characterization, functional analysis, development and production of genetic probes, and the design of neuropeptide derivatives for cellular neurobiology as well as the potential therapeutic applications. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to follow, Neuropeptides: Methods and Protocols is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, and electrophysiologists that are currently active in the field or are willing to enter this exciting and still expanding area of neurobiology.
Since the early days, the field of neuropeptide biology has dramatically widened, and today the ultimate frontiers in neuropeptide research lie in the development of pharmacologically active compounds that are capable of crossing the blood-brain barrier to exert their biological role(s) in vivo and in the construction of genetic vectors to be employed in gene therapy. Neuropeptides: Methods and Protocols presents a readily reproducible collection of established and emerging techniques for neuropeptide research as contributed by expert researchers in the field. The detailed methods presented cover subjects such as immunocytochemical localization, biochemical characterization, functional analysis, development and production of genetic probes, and the design of neuropeptide derivatives for cellular neurobiology as well as the potential therapeutic applications. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to follow, Neuropeptides: Methods and Protocols is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, and electrophysiologists that are currently active in the field or are willing to enter this exciting and still expanding area of neurobiology.
Zusammenfassung
Features contributions from scientists with direct experience in one or more of the fields of neuropeptide research
Include detailed protocols relevant to a wide range of scientists
Provides key tips and expert implementation advice to ensure successful results
Inhaltsverzeichnis
1. What Are Neuropeptides? J. Peter H. Burbach 2. Neuropeptide Localization in Non Mammalian Vertebrates Paolo de Girolamo and Carla Lucini 3. Combined Light and Electron Microscopic Visualization of Neuropeptides and Their Receptors in Central Neurons Chiara Salio, Laura Lossi, and Adalberto Merighi 4. Neuropeptide RNA Localization in Tissue Sections Marc Landry, Chérine Abdel Salam, and Marie Moftah 5. Intron-Specific Neuropeptide Probes Harold Gainer, Todd A. Ponzio, Chunmei Yue, and Makoto Kawasaki 6. Direct In Situ RT-PCR Laura Lossi, Graziana Gambino, Chiara Salio, and Adalberto Merighi 7. Laser Capture Microdissection and Quantitative-PCR Analysis Sarah J. Paulsen and Leif K. Larsen 8. Visualization of Peptide Secretory Vesicles in Living Nerve Cells Park J. Joshua and Loh Y. Peng 9. Fluorescence Imaging with Single-Molecule Sensitivity and Fluorescence Correlation Spectroscopy (FCS) of Cell-Penetrating Neuropeptides Vladana Vukojevi¿, Astrid Gräslund, and Georgy Bakalkin 10. Analysis of Neuro-Immune Interactions by an In Vitro Co-Culture Approach Tadahide Furuno and Mamoru Nakanishi 11. Electrophysiology Zhi-Qing David Xu 12. Localization of Neuropeptides by Radioimmunoassay Fred Nyberg and Mathias Hallberg 13. Reversed-Phase HPLC and Hyphenated Analytical Strategies for Peptidomics Anne-Marie Hesse, Sega Ndiaye, and Joelle Vinh 14. Neuropeptidomics: Mass Spectrometry-Based Qualitative and Quantitative Analysis Ping Yin, Xiaowen Hou, Elena V. Romanova, and Jonathan V. Sweedler 15. Suppression Subtractive Hybridization Mohamed T. Ghorbel and David Murphy 16. Neuropeptide Microdialysis in Free-Moving Animals Tetsuya Kushikata and Kazuyoshi Hirota 17. Antibody Microprobes for Detecting Neuropeptide Release Carole A. Williams and Arthur W. Duggan 18. Neuropeptidases Manuel Ramírez, Isabel Prieto, Inmaculada Banegas, Ana B. Segarra, and Francisco Alba 19. Neuropeptide Autoantibodies Assay Sergueï O. Fetissov 20. Intranasal Delivery of Neuropeptides Michael C. Veronesi, Daniel J. Kubek, and Michael J. Kubek 21. Prodrug Design for Brain Delivery of Small- and Medium-Sized Neuropeptides Katalin Prokai-Tatrai and Laszlo Prokai 22. Measurement of Phosphorothioate Oligodeoxynucleotide Antisense Transport Across the Blood-Brain Barrier William A. Banks 23. Liposome-Encapsulated Neuropeptides for Site-Specific Microinjection Frédéric Frézard, Robson A.S. dos Santos, and Marco A.P. Fontes 24. Recombinant Adeno-Associated Viral Vectors Marijke W.A. de Backer, Keith M. Garner, Mieneke C.M. Luijendijk, and Roger A.H. Adan 25. De-Orphanizing G Protein-Coupled Receptors by a Calcium Mobilization Assay Isabel Beets, Marleen Lindemans, Tom Janssen, and Peter Verleyen
[Mehr]
