Provides researchers with innovative methodologies for plant pathogen diagnostics

Written by experts from internationally renowned institutions

Cross-cutting approach to diagnostics, including molecular, serological, and traditional methodologies

All classes of plant pathogen covered in methodologies

Provides methods for crop plant genetic validation

Includes supplementary material: [...]

Bioassay for Diagnosis of Plant Viruses.- Development of Enzyme Linked, Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection.- Immunisation strategies for Antibody Production.- Preparation of Immunogens and Production of Antibodies.- Enzyme-Linked Immunosorbent Assay Detection of Ralstonia solanacearum in Potatoes: The South African Experience.- Production of Monoclonal Antibodies to Plant Pathogens.- Enzyme-Linked Immunosorbent Assay for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus).- Indirect Immunofluorescence Microscopy for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Ralstonia solanacearum).- Detection of Four Major Bacterial Potato Pathogens.- Erwinia amylovora: Modern Methods for Detection and Differentiation.- The use of Fluorescent in situ Hybridisation in Plant Fungal Identification and Genotyping.- Use of Molecular Methods for the Detection of Fungal Spores.- Identification of Phytophthora fragariae var. rubi by PCR.- Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani.- Immunocapture-PCR for Plant Virus Detection.- Multiplex Polymerase Chain Reaction (PCR) and Real-time Multiplex PCR for the Simultaneous Detection of Plant Viruses.- Fluorescent-based Techniques for Viral Detection, Quantification andCharacterisation.- Analysis of Population Structures of Viral Isolates using Single StrandConformation Polymorphism (SSCP) Method.- Direct Detection of Plant Viruses in Potato Tubers using Real-time PCR.- An Accelerated Soil Bait Assay for the Detection of Potato Mop Top Virus in Agricultural Soil.- Detection of Phytoplasmas of Temperate Fruit Trees.- PCR Detection of Potato Cyst Nematode.- Potato Cultivar Genome Analysis.- Barley Variety Identification Using SSRs.